|
|
試劑名稱
|
配制試劑方法
|
規(guī)格
|
價(jià)格
|
|
1
|
1.5M
Tris-HCl (pH8.8, 200ml)
|
Tris
base
|
36.34g
|
|
¥0.00
|
|
|
ddH2O
|
160ml
|
|
¥0.00
|
|
|
用HCl調(diào)至pH8.8后，定容至
|
200ml
|
200ml
|
¥0.00
|
|
2
|
1.0M
Tris-HCl (pH6.8, 250ml)
|
Tris
base
|
30.9g
|
|
¥0.00
|
|
|
ddH2O
|
200ml
|
|
¥0.00
|
|
|
用HCl調(diào)至pH6.8后，定容至
|
250ml
|
250ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
3
|
6*上樣Buffer
|
4*Tris-HCl(pH6.8)
|
7ml
|
|
¥0.00
|
|
|
Glycerol
(甘油)
|
3ml
|
|
¥0.00
|
|
|
SDS
|
1g
|
|
¥0.00
|
|
|
DTT
|
0.93g
|
|
¥0.00
|
|
|
bromphenol
blue
|
1.2mg
|
|
¥0.00
|
|
|
H2O定容至
|
10ml
|
10ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
4
|
10%APS(過硫酸銨）
|
過硫酸銨
|
0.1g
|
|
¥0.00
|
|
|
ddH2O
|
1ml
|
1mlX10支
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
5
|
10%SDS
|
SDS
|
10g
|
|
¥0.00
|
|
|
加熱至68度溶解，HCl調(diào)pH7.2
|
|
|
¥0.00
|
|
|
ddH2O定容至100ml 滅菌
|
100ml
|
100ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
6
|
10*TBS
|
Tris
base
|
24.2g
|
|
¥0.00
|
|
|
NaCl
|
80g
|
|
¥0.00
|
|
|
HCl調(diào)pH7.6
|
|
|
¥0.00
|
|
|
ddH2O定容至
|
1L
|
1L
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
7
|
Cocktail
RIPA
|
|
1ml
|
1ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
8
|
10*Running
buffer
|
Tris
base
|
30.2g
|
|
¥0.00
|
|
|
甘氨酸
|
188g
|
|
¥0.00
|
|
|
10%SDS
|
100ml
|
|
¥0.00
|
|
|
ddH2O定容至
|
1L
|
1L
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
9
|
10*轉(zhuǎn)膜Buffer(不含甲醇)
|
Tris
base
|
30.3g
|
|
¥0.00
|
|
|
甘氨酸
|
144g
|
|
¥0.00
|
|
|
ddH2O定容至
|
1L
|
1L
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
10
|
5XTBE
|
Tris
base
|
54g
|
|
¥0.00
|
|
|
Na2EDTA.H2O
|
3.72g
|
|
¥0.00
|
|
|
硼酸
|
27.5g
|
|
¥0.00
|
|
|
ddH2O定容至
|
1L
|
1L
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
11
|
PMSF
(100mg/ml)
|
PMSF
|
17.4mg
|
|
¥0.00
|
|
|
異丙醇
|
1ml
|
1ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
12
|
口腔漱口液保存液
|
NaCl
|
13.2g
|
|
¥0.00
|
|
|
檸檬酸鈉
|
6.6g
|
|
¥0.00
|
|
|
氯仿
|
15mL
|
|
¥0.00
|
|
|
伊紅溶液（HE染色的伊紅）
|
3-5滴
|
|
¥0.00
|
|
|
ddH2O定容至
|
1L
|
1L
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
13
|
0.5M
EDTA（pH8.0）,250ml
|
EDTA·2Na
|
42.27g
|
|
¥0.00
|
|
NaOH
|
2g
|
|
¥0.00
|
|
ddH2O定容至
|
250ml
|
250ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
14
|
1M
Tris-HCl(pH8.0),1L
|
Tris
base
|
121g
|
|
¥0.00
|
|
ddH2O
|
800ml
|
|
¥0.00
|
|
濃HCl調(diào)pH至8.0，后定容至
|
1L
|
1L
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
15
|
糞便DNA保存液
|
1M
Tris-HCl
|
5 ml
|
|
¥0.00
|
|
0.5M EDTA
|
100 ml
|
|
¥0.00
|
|
SDS
|
2.5g
|
|
¥0.00
|
|
ddH2O定容至
|
500ml
|
500ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
16
|
蛋白和封閉液防腐試劑
|
|
|
|
¥0.00
|
|
任意蛋白或封閉液
|
X
|
|
¥0.00
|
|
0.1% sodium azide（疊氮鈉）
|
1g
|
|
¥0.00
|
|
0.1%Gelatin
|
1g
|
|
¥0.00
|
|
用PBS定容至
|
1L
|
|
¥0.00
|
|
防止細(xì)菌生長，該試劑放在4度即可。
|
|
1L
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
17
|
蛋白酶K緩沖液
|
1M
Tris-HCl
|
25ml
|
|
¥0.00
|
|
0.5M EDTA
|
50ml
|
|
¥0.00
|
|
ddH2O定容至
|
500ml
|
500ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
18
|
20mg/ml
蛋白酶K
|
蛋白酶K緩沖液
溶解
|
5ml
|
|
¥0.00
|
|
滅菌甘油
|
5ml
|
|
¥0.00
|
|
終體積
|
10mL
|
10ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
19
|
F.A.A固定液，標(biāo)準(zhǔn)固定液,萬能固定液。
|
福爾馬林(38%甲醛)
|
5mL
|
|
¥0.00
|
|
冰醋酸
|
5mL
|
|
¥0.00
|
|
70%酒精
|
90mL
|
|
¥0.00
|
|
甘油
|
5mL
|
|
¥0.00
|
|
|
適用于根,莖,葉,花藥,子房組織切片.在植物形態(tài)解剖研究上應(yīng)用極廣,此固定液最大優(yōu)點(diǎn)是兼有保存劑作用,對(duì)染色體的觀察效果較差。幼嫩材料用50%酒精代替70%酒精,防材料收縮;甘油(丙三醇)以防蒸發(fā)和材料變硬.
|
|
105ml
|
¥0.00
|
|
20
|
TNT
buffer
|
Tris
base 0.1M
|
12.11g
|
|
¥0.00
|
|
|
NaCl 150mM
|
8.77g
|
|
¥0.00
|
|
|
Triton-100 0.3%
|
3ml
|
|
¥0.00
|
|
|
可增加2mM左旋咪唑Tetramisde
hydrochloride）
|
|
|
¥0.00
|
|
|
加純水
|
1000ml
|
|
¥0.00
|
|
21
|
4-IPBA（TSA）
|
4-IPBA 20mg/ml
|
20mg
|
|
¥0.00
|
|
|
加純水
|
1ml
|
1ml
|
¥0.00
|
|
22
|
Azide
solution
|
Azide
100mM
|
10ml
|
|
¥0.00
|
|
23
|
NBT（50mg/ml）(4.5ul/ml)
|
NBT
50mg
|
1ml
|
|
¥0.00
|
|
|
700ul
DMF +300ul 水
|
1ml
|
¥0.00
|
|
24
|
BCIP
(50mg/ml)(3.5ul/ml)
|
50mg
|
1ml
|
|
¥0.00
|
|
|
DMF
|
1ml
|
¥0.00
|
|
25
|
NBT/BCIP
development buffer(TMN buffer)
|
1M
Tris pH=9.5 100ml
|
|
|
¥0.00
|
|
|
5M
NaCl 20ml
|
|
|
¥0.00
|
|
|
1M
MgCl2 50ml
|
|
|
¥0.00
|
|
|
10%
Tween-20 10ml
|
|
|
¥0.00
|
|
|
補(bǔ)水1000ml
|
|
1000ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
26
|
10%
PVA
|
100g
|
1000ml
|
|
¥0.00
|
|
|
800ml水
|
|
|
¥0.00
|
|
|
補(bǔ)水至1000ml
|
|
1000ml
|
¥0.00
|
|
27
|
20XSSC(粉末
1L)
|
NaCl
175.3g
|
|
|
¥0.00
|
|
|
檸檬酸鈉*2H2O
88.2g
|
|
1L
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
28
|
5%
Pepsin
|
pepsin
|
1g
|
|
¥0.00
|
|
|
H2O
|
20ml
|
20ml
|
¥0.00
|
|
29
|
20ng/ml
DAPI
|
DAPI+抗淬滅劑
|
1mg
|
|
¥0.00
|
|
|
H2O
|
50ml
|
50ml
|
¥0.00
|
|
30
|
DNA
probe HY buffer
|
DNA
probe
|
2.5uL
|
|
¥0.00
|
|
|
20XSSC
|
1ul
|
|
¥0.00
|
|
|
去離子甲酰胺
|
6.5uL
|
10ul/反應(yīng)
|
¥0.00
|
|
31
|
NTE
buffer(Stop buffer NBTBCIP)
|
0.5M
NaCl
|
|
|
¥0.00
|
|
|
0.01M
Tris
|
|
|
¥0.00
|
|
|
0.005M
EDTA* Na2
|
|
|
¥0.00
|
|
|
0.0005%
Tween-20
|
|
|
¥0.00
|
|
32
|
2M 甘氨酸
|
1.5g
甘氨酸
|
1.5g
|
|
¥0.00
|
|
|
純水
|
10ml
|
10ml
|
¥0.00
|
|
|
0.2 M
Sodium Bicarbonate buffer
|
NaHCO3
|
168mg=0.168g
|
|
¥0.00
|
|
|
純水
|
10ml
|
|
¥0.00
|
|
|
調(diào)節(jié)到pH=8.5-9.5
|
|
10ml
|
¥0.00
|
|
33
|
1 M
Sodium Bicarbonate buffer
|
NaHCO3
|
84.1mg
|
|
¥0.00
|
|
|
純水
|
1000uL
|
|
¥0.00
|
|
|
調(diào)節(jié)到pH=8.5-9.5
|
|
1ml
|
¥0.00
|
|
34
|
1 M
NaOH
|
NaOH
|
100mg
|
|
¥0.00
|
|
|
純水
|
2.5ml
|
|
¥0.00
|
|
|
用塑料容器、室溫
|
|
2.5ml
|
¥0.00
|
|
35
|
1 M
HCl
|
純水
|
2.5ml
|
|
¥0.00
|
|
|
hydrochloric
acid
|
91.5mg
|
|
¥0.00
|
|
|
用塑料容器、室溫
|
|
2.5ml
|
¥0.00
|
|
36
|
MES
(0.5 M, pH 6)
|
MES
free acid (195.24 g/mol)
|
9.762 g
|
|
¥0.00
|
|
|
10N
NaOH
|
1.36
|
|
¥0.00
|
|
|
純水補(bǔ)充到100ml
|
100ml
|
100ml
|
¥0.00
|
|
37
|
Sodium
Borate Buffer (1 M, pH 8.5)
|
Boric
Acid (H3BO3) (61.83 g/mol)
|
6.183 g
|
|
¥0.00
|
|
|
NaOH
(40.00 g/mol)
|
1 g
|
|
¥0.00
|
|
|
純水
|
100ml
|
100ml
|
¥0.00
|
|
38
|
MOPS
Buffer (10X) (0.2 M, pH 7)
|
MOPS
free acid (mw: 209.26 g/mol)
|
4.186
g
|
|
¥0.00
|
|
|
Sodium
Acetate (mw: 82.03 g/mol)
|
410
mg
|
|
¥0.00
|
|
|
Na2EDTA (mw: 372.24 g/mol)
|
372
mg
|
|
¥0.00
|
|
|
純水
|
100ml
|
100ml
|
¥0.00
|
|
39
|
Maleic
Acid (1 M, pH 7.5)
|
Maleic
Acid (mw: 116.07 g/mol)
|
11.61
g
|
|
¥0.00
|
|
|
NaCl
(mw: 58.44 g/mol)
|
8.76
g
|
|
¥0.00
|
|
|
NaOH
(mw: 40.00 g/mol)
|
4
g
|
|
¥0.00
|
|
|
純水
|
100ml
|
100ml
|
¥0.00
|
|
40
|
CAPS
(0.5 M, pH 10.4)
|
CAPS
(mw: 221.32 g/mol)
|
11.066
g
|
|
¥0.00
|
|
|
純水
|
100ml
|
100ml
|
¥0.00
|
|
41
|
Carbonate-Bicarbonate
Buffer (pH 9.5，0.1M）
|
Sodium
bicarbonate (84.01 g/mol)
|
623 mg
|
|
¥0.00
|
|
|
Sodium
carbonate (anhydrous) (105.99 g/mol)
|
274 mg
|
|
¥0.00
|
|
|
純水
|
100ml
|
100ml
|
¥0.00
|
|
42
|
Carbonate-Bicarbonate
Buffer (pH 9.2，0.2M）
|
Sodium
bicarbonate (84.01 g/mol)
|
1.529 g
|
|
¥0.00
|
|
|
Sodium
carbonate (anhydrous) (105.99 g/mol)
|
191 mg
|
|
¥0.00
|
|
|
純水
|
100ml
|
|
¥0.00
|
|
43
|
Tris
Buffer (1 M, pH 7.2)
|
Tris
base (mw: 121.14 g/mol)
|
60.57
g
|
100ml
|
¥0.00
|
|
|
Adjust
pH using HCl pH=7.2
|
|
|
¥0.00
|
|
44
|
1M DTT
|
DTT
|
3.09g
|
|
¥0.00
|
|
|
0.01M
醋酸鈉（pH=5.2）
|
20ml
|
|
¥0.00
|
|
|
0.22uM過濾，分成2ml小份。-20保存
|
|
20ml
|
¥0.00
|
|
45
|
琥珀酸溶液
|
琥珀酸試劑分子生物純度 WM：118.09
|
11.8
|
|
¥0.00
|
|
|
純水
|
500ml
|
|
¥0.00
|
|
|
調(diào)整到
pH=5.5
|
|
|
¥0.00
|
|
|
定容至500ml
|
|
500ml
|
¥0.00
|
|
46
|
Chelex溶液
|
Chelex溶液
DNA提取專用
|
100ml
|
100ml
|
¥0.00
|
|
47
|
Acetate
Buffer （醋酸鈉溶液）
|
Sodium
Acetate (mw: 82.03 g/mol)
|
577
mg
|
|
¥0.00
|
|
|
Acetic
Acid (mw: 60.05 g/mol)
|
178
mg
|
|
¥0.00
|
|
|
pH值調(diào)整到pH
5.0-5.2
|
|
100ml
|
¥0.00
|
|
48
|
Citrate
Buffer (0.1 M, pH 6.0)
|
Sodium
Citrate dihydrate (mw: 294.10 g/mol)
|
2.427
g
|
|
¥0.00
|
|
|
Citric
Acid (mw: 192.12 g/mol)
|
336
mg
|
|
¥0.00
|
|
|
pH值調(diào)整到pH
6.0
|
|
100ml
|
¥0.00
|
|
49
|
二乙醇胺溶液
|
diethanolamine
(mw: 105.14 g/mol)
|
10.514
g
|
|
¥0.00
|
|
|
|
MgCl2-6H2O (mw: 203.30 g/mol)
|
10
mg
|
|
¥0.00
|
|
|
|
pH值調(diào)整到pH
9.8
|
|
100ml
|
¥0.00
|
|
50
|
Imidazole-HCl
Buffer (0.05 M, pH 7.0)
|
Imidazole
(mw: 68.08 g/mol)
|
340
mg
|
|
¥0.00
|
|
|
HCl
(mw: 36.46 g/mol)
|
89
mg
|
100ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
51
|
TAE (1
M, pH 8.5，50X)
|
Tris
base (mw: 121.14 g/mol)
|
121g
|
|
¥0.00
|
|
|
Disodium
EDTA (mw: 372.24 g/mol)
|
9.305g
|
|
¥0.00
|
|
|
Acetic
Acid (mw: 60.05 g/mol)
|
29.978g
|
|
¥0.00
|
|
|
加水調(diào)節(jié)到500ml
|
500ml
|
500ml
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
52
|
TE
Buffer 10X
|
Tris-Cl
(desired pH) (mw: 157.594 g/mol)
|
1.576
g
|
|
¥0.00
|
|
|
EDTA
(pH 8) (mw: 292.24 g/mol)
|
292
mg
|
|
¥0.00
|
|
|
調(diào)整到pH7.5
或8.0
|
100ml
|
100ml
|
¥0.00
|
|
53
|
原位雜交RNA修復(fù)液
|
Trisodium
citrate
|
29.4
|
1000ml
|
¥0.00
|
|
|
trisodium
citrate
|
5ml
|
|
¥0.00
|
|
|
|
|
|
|
|
|
|
溶液名稱
|
組成
|
濃度
|
pH值
|
¥0.00
|
|
54
|
細(xì)胞穿透液，pH7.5
|
HEPES
|
15mM
|
|
¥0.00
|
|
|
KCl
|
50mM
|
|
¥0.00
|
|
|
MgCl2
|
1mM
|
|
¥0.00
|
|
|
EGTA
|
1mM
|
|
¥0.00
|
|
|
DTT
|
1mM
|
|
¥0.00
|
|
|
Saponin
|
0.01%
|
|
¥0.00
|
|
|
Triton
|
0.02%
|
pH7.5
|
¥0.00
|
|
55
|
0.5M EGTA
|
EGTA
|
0.5M
|
|
¥0.00
|
|
|
|
|
pH8.0
|
¥0.00
|
|
|
緩沖液
|
使用液
|
儲(chǔ)備液
|
|
¥0.00
|
|
56
|
Tris-乙醇（TAE）
|
1×:0.04M Tris-乙酸
|
50×:242g Tris堿
|
|
¥0.00
|
|
|
1×: 0.001M EDTA
|
50×:57.1ml 冰乙酸
|
|
¥0.00
|
|
|
|
50×:100ml 0.5M
EDTA(PH8.0)
|
|
¥0.00
|
|
57
|
Tris-磷酸（TPE）
|
1×：0.09M Tris-磷酸
|
10×：108g Tris堿
|
|
¥0.00
|
|
|
0.002M EDTA
|
15.5ml
85%冰乙酸（1.679g/ml)
|
|
¥0.00
|
|
|
|
40ml 0.5M EDTA(PH8.0)
|
|
¥0.00
|
|
58
|
Tris-硼酸（TBE）
|
0.5×:0.045 Tris-硼酸
|
5×:54g Tirs堿
|
|
¥0.00
|
|
|
0.5×:0.001M EDTA
|
5×:27.5g硼酸
|
|
¥0.00
|
|
|
|
5×: 20ml 0.5M
EDTA(PH8.0)
|
|
¥0.00
|
|
59
|
堿性緩沖液
|
1×:50mM NaOH
|
1×:5ml 10mol NaOH
|
|
¥0.00
|
|
|
1×:1M EDTA
|
1×:2ml 0.5M
EDTA(PH8.0)
|
|
¥0.00
|
|
60
|
Tris-甘氨酸
|
1×:25mM Tris
|
5×：15.1g Tirs堿
|
|
¥0.00
|
|
|
1×:250mM 甘氨酸
|
94g甘氨酸（電泳級(jí)）(PH8.3)
|
|
¥0.00
|
|
|
1×: 0.1%SDS
|
50ml 10%SDS（電泳級(jí)）
|
|
¥0.00
|
|
61
|
HEPESS
Buffer （pH= 7.5）
|
配1M溶液，稱量Hepes（238.30
g/mol）
|
23.83g
|
|
¥0.00
|
|
|
|
量取80ml純凈水，定容100ml
|
100ml
|
|
¥0.00
|
|
|
|
|
|
|
¥0.00
|
|
備注：外顯子生物實(shí)驗(yàn)室溶液中心，能24小時(shí)送達(dá)廣州市范圍內(nèi)實(shí)驗(yàn)室，質(zhì)量保證。價(jià)格在50-150元內(nèi)。
|
